S Saravani; N Tanideh; M Fazeli; N Azarpira; E Nadimi; M Lotfi; Ayda Iraji; Sh Zareh
Abstract
Background: Osteoarthritis (OA) recognized, as degenerative arthritis. Synthetic drugs available to treat the OA have, the dangerous side effects and low efficacy. Therefore, using of herbal medicines is an important component of alternative and complementary medicine, Mesenchymal stem cells have ...
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Background: Osteoarthritis (OA) recognized, as degenerative arthritis. Synthetic drugs available to treat the OA have, the dangerous side effects and low efficacy. Therefore, using of herbal medicines is an important component of alternative and complementary medicine, Mesenchymal stem cells have a special site in the treatment of diseases. drugs.Psidium guajava Leaves used as antioxidant, anti-inflammatory antinociceptive and analgesic. The goals of scientific research of this study were to the evaluate the regeneration articular cartilage effect of injection of the hydroalcoholic extract of leave of Psidium Guajava with Stem Cell into the knee joints of rats.
Methods: Firstly, ninety male rats were choosen, and collagenase type II was prepared for an intra-articular knee injection, for them to induce OA in the animal models. Then, they were divided into nine treatment groups. (N = 10): (a) Sham, (b) Control, (c) injection of hyaluronic Acid intra-articular, (d) Injections of Stem Cell intra-articular, (e) Injections of PRP, (f): Injections of the extract Psidium guajava leaves (g) Injections of the extract Psidium guajava leave with PRP, (h) Injections of the extract Psidium guajava leaves with Stem Cell (i) Injections of the extract Psidium guajava leaves with Stem Cell and PRP. After 5 months, the results were assessments by a Radiologist and Histopathologist.
Results: Radiological and histopathological findings were in which the improvement of the articular cartilage was better in the leaves with Stem Cell and PRP compared with the control group(P<0.05).
Conclusions: It seems that Psidium guajava leaves can be suggested as an effective complementary and alternative treatment for OA of the knee. Because it has a lot of polyphenol compounds.
giti Borzooeian; Nader Tanideh; Mehrzad Lotfi; Maral Mokhtari; Habibollah Nazem; Mahsa Sani; Mahin Salman-nezhad; omid koohi-hosseinabadi; sajad daneshi
Abstract
Background: Osteoarthritis is a progressive joint disease. Mastic fruit has antioxidant and anti-inflammatory properties. Bone marrow stem cells have the ability to differentiate into chondrocyte. The purpose of this study is evaluation the effect of Pistacia atlantica hydro alcoholic extract with bone ...
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Background: Osteoarthritis is a progressive joint disease. Mastic fruit has antioxidant and anti-inflammatory properties. Bone marrow stem cells have the ability to differentiate into chondrocyte. The purpose of this study is evaluation the effect of Pistacia atlantica hydro alcoholic extract with bone marrow- derived stem cells on induced knee osteoarthritis in rat.
Methods: Firstly, 50 male Wistar rats were divided into 5 groups of 10. Then, on days zero and 3, fifty μl collagenase type 2 under anesthesia was injected in the left knee. After 10 weeks, osteoarthritis was induced and rats based on the type of treatment were divided into 5 groups including: control, treatment by stem cell and Pistacia atlantica hydro alcoholic extract, Pistacia atlantica hydro alcoholic extract therapy, stem cell therapy and treatment with hyalgan. At the end of study, radiographic and histopathology surveys and also measured of antioxidant properties as MDA-TAC was done.
Results: After 12 weeks of treatment, radiographic and histopathological evaluation showed that the results of the cell with extract group was significantly different compared to other treatment groups and the control group (P <0.05). Also, based on biochemical evaluation of MDA-TAC in cell with extract group significant reduction in oxidative stress is observed compared to other treatment groups and control (P <0.05).
Conclusions: It seems that Pistacia atlantica hydro alcoholic extract with bone marrow- derived stem cells are effective in knee cartilage repair.
Mohammad Jafar Emami; Fereidoon Mojtahed Jaberi; Negar Azarpira; Amir Reza Vosoughi; Nader Tanideh, DVM, MPH, PhD
Abstract
Background: Prevention of arthrofibrosis by different drugs and surgical techniques is an essential issue in modern orthopaedics. This study investigated the effect of bevacizumab on intra-articular fibrosis in a rabbit animal model.Methods: Arthrofibrosis was induced in right stifle joint of thirty ...
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Background: Prevention of arthrofibrosis by different drugs and surgical techniques is an essential issue in modern orthopaedics. This study investigated the effect of bevacizumab on intra-articular fibrosis in a rabbit animal model.Methods: Arthrofibrosis was induced in right stifle joint of thirty male white rabbits by removing the cortical bone of femoral condyle in medial gutter under general anesthesia. The rabbits were randomly divided into three equal groups. The control group received intra-articular injection of saline the "one-injection" group received a single dose of bevacizumab (2.5 mg/kg), and the" two-injection" group received two intra-articular injections on the day of operation and 14 days later. Forty five days after the surgery, the animals were sacrificed. The severity of fibrosis was assessed by range of motion of the stifles, macroscopic adhesion score, and histopathologic variables.Results: Although no statistical difference was seen between the control group and the "one-injection" group in terms of range of motion and macroscopic evaluation, the histopathologic variables in prevention of arthrofibrosis, except for the granulation tissue, were significantly better in the "one-injection" group. The "two-injection" group in comparison to the "one-injection" group was better not only in range of motion evaluation (p < /em>=.001) and visual adhesion score (p < /em>=.012), but also in most of the histopathologic variables, except for the cell count and granulation tissue.Conclusions: Two injections of bevacizumab for prevention of arthrofibrosis resulted in better outcome than single injection.
